Construction of gene-targeting vectors by recombineering

摘要

Recombineering is a technology that utilizes the efficient homologous recombination functions encoded by λ phage to manipulate DNA in Escherichia coli. Construction of knockout vectors has been greatly facilitated by recombineering as it allows one to choose any genomic region to manipulate. We describe here an efficient recombineering-based protocol for making mouse conditional knockout targeting vectors. © 2009 Humana Press, a part of Springer Science+Business Media, LLC.